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anti slc3a2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti slc3a2
    Anti Slc3a2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 834 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+slc3a2/HIF-1alpha+XP+Rabbit+mAb/pm41894392-381-15-16
    Average 97 stars, based on 834 article reviews
    anti slc3a2 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    other:

    Article Title: SLC13A3 is a major effector downstream of activated β-catenin in liver cancer pathogenesis
    Article Snippet: Several critical antibodies such as SLC13A3, -Catenin, c-MYC and SLC7A5 were validated using either over-expressing or knockdown cell lines in this study. anti-SLC13A3 (Abcam, #Ab56017): Identification of 54 kD band in WB using whole Jurkat cell lysate. anti--Catenin (CST, #8480S): Identification of 92 kD (wild-Type) and truncated band (Mutant) in WB using HeLa cell lysate. anti-pmTOR and anti-mTOR (CST, #5536S, #2983S): Identification of 289 kD in WB using NIH/3T3 cell lysate. anti-pp70s6k (CST, #9204S): Identification of 85 and 70 kD in WB using 293 cell lysate. anti-P62 (CST, #8025S): Identification of 62 kD in WB using HeLa cell lysate. anti-ATG5 (CST, #12994S): Identification of 55 kD in WB using different cells lysates. anti-Cleaved casapase-3 (CST, #9664S): Identification of 17 or 19 kD in WB using C6, NIH/3T3 and Jurkat cell lysates. anti-LC3B (CST, #3868S): Identification of 14 and 16 kD in WB using HCT116 cell lysate. anti-FTH1 (Santa Cruz, #sc-376594): Identification of 21 kD in WB using HeLa, HepG2 and MCF7 cell lysates. anti-TfR1 (Abcam, #ab214039): Identification of 85 kD in WB using different human or mouse cells lysate. anti-SLC7A5 (Beijing Biosysthesis, #bs-10125R): Identification of 55 kD in WB using rat brain lysate. anti-SLC3A2 (Cell Signaling Technology, #13180S):Identification of 75 kD in WB using HeLa cell lysate. anti-GPX4(ABclonal Technology Co.,Ltd., A13309):Identification of 22 kD in WB using Jurkat cell lysate anti-Na+, K+ ATPase (Cell Signaling Technology, #3010S):Identification of 100 kD in WB using MCF-7, COS, A431,C6,C2C12 cell lysates. anti-c-MYC (CST, #18583S): Identification of 57 kD in WB using different cell lysates. anti--actin (CST, #4970S): Identification of 45 kD in WB using different cell lysates. anti-MYC-tag (CST, #2276S): Identification of 100 kD in WB using transfected 293 cell lysate. anti-HA-tag (CST, #3724S): Identification of 60 kD in WB using transfected HeLa cell lysate.

    Incubation:

    Article Title: Disulfidptosis-related gene SLC3A2: a novel prognostic biomarker in nasopharyngeal carcinoma and head and neck squamous cell carcinoma
    Article Snippet: The isolated proteins were transferred from the gel to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore) and blocked with 5% skim milk for one hour at room temperature. .. The blots were cut and then incubated with the primary antibodies of anti-SLC3A2 (CST-13180S, 1:1,000 dilution), rabbit anti-GAPDH (Servicebio-GB15004, 1:3,000 dilution) respectively at 4°C overnight. .. After three times of washing with TBST, the membranes were incubated with HRP-conjugated goat anti-rabbit IgG (Beyotime-A0208, 1:3,000 dilution) for an hour at room temperature.

    Article Title: Anaplastic Lymphoma Kinase signaling stabilizes SLC3A2 expression via MARCH11 to promote neuroblastoma cell growth.
    Article Snippet: NB1 cells were treated with ALKAL2 (1 μg/ml) or/and lorlatinib (30 nM) or/ and MG132 (10 μM), CLB-GE and CLB-BAR cells were treated with lorlatinib (30 nM) for 24 h prior to lysis with IP buffer (150mM NaCl, 50 mM Tris-HCl, 1% Triton X-100, 1% glycerol) containing protease inhibitor cocktail (#04693132001, Roche). .. Either 3 μg anti-ALK (#3633, CST), anti-SLC3A2 (#13180, CST), anti-phospho-Tyrosine (p-Y-1000, #8954, CST) or isotype control (#AB-105-C, R&D systems) antibody was added to 300 μg of cell lysate for overnight rotation at 4 °C, followed by incubation with 25 μl Protein G Sepharose 4 Fast Flow for an additional 2 h. Samples were washed twice with high salt IP buffer (500mM NaCl, 50 mM Tris-HCl, 1% Triton X-100, 1% glycerol) and then washed once with IP buffer (150mM NaCl, 50 mM Tris-HCl, 1% Triton X-100, 1% glycerol). ..

    Article Title: Anaplastic Lymphoma Kinase signaling stabilizes SLC3A2 expression via MARCH11 to promote neuroblastoma cell growth
    Article Snippet: NB1 cells were treated with ALKAL2 (1 μg/ml) or/and lorlatinib (30 nM) or/and MG132 (10 μM), CLB-GE and CLB-BAR cells were treated with lorlatinib (30 nM) for 24 h prior to lysis with IP buffer (150 mM NaCl, 50 mM Tris-HCl, 1% Triton X-100, 1% glycerol) containing protease inhibitor cocktail (#04693132001, Roche). .. Either 3 μg anti-ALK (#3633, CST), anti-SLC3A2 (#13180, CST), anti-phospho-Tyrosine (p-Y-1000, #8954, CST) or isotype control (#AB-105-C, R&D systems) antibody was added to 300 μg of cell lysate for overnight rotation at 4 °C, followed by incubation with 25 μl Protein G Sepharose 4 Fast Flow for an additional 2 h. Samples were washed twice with high salt IP buffer (500 mM NaCl, 50 mM Tris-HCl, 1% Triton X-100, 1% glycerol) and then washed once with IP buffer (150 mM NaCl, 50 mM Tris-HCl, 1% Triton X-100, 1% glycerol). ..

    Western Blot:

    Article Title: Phase Separation of NFIB Suppresses SLC3A2-Mediated Ferroptosis in Castration-Resistant Prostate Cancer.
    Article Snippet: .. The primary antibodies used in Western blotting included anti-NFIB (1:1000, Abcam), anti-SLC3A2 (1:1000, CST), anti-SLC7A11 (1:1000, CST), anti-GAPDH (1:5000, proteintech), anti-Ace-lys (1:1000, CST). ..

    Article Title: Metformin Reprograms Tryptophan Metabolism to Stimulate CD8+ T-cell Function in Colorectal Cancer
    Article Snippet: .. The following antibodies were used for Western blotting: anti-SLC3A2 (47213S, Cell Signaling Technology), anti-SLC36A4 (NBP2–50237, Novus Biologicals), anti-SLC1A5 (8057, Cell Signaling Technology), anti-SLC7A5 (5347, Cell Signaling Technology), anti-IDO1 (MAB5412, Sigma-Aldrich), anti-MYC (ab185656, Abcam), and anti-AHR (sc-133088, Santa Cruz Biotechnology). .. For intracellular cytokine staining, the cells were first activated by a leukocyte activation cocktail (BD Biosciences).

    Control:

    Article Title: Anaplastic Lymphoma Kinase signaling stabilizes SLC3A2 expression via MARCH11 to promote neuroblastoma cell growth.
    Article Snippet: NB1 cells were treated with ALKAL2 (1 μg/ml) or/and lorlatinib (30 nM) or/ and MG132 (10 μM), CLB-GE and CLB-BAR cells were treated with lorlatinib (30 nM) for 24 h prior to lysis with IP buffer (150mM NaCl, 50 mM Tris-HCl, 1% Triton X-100, 1% glycerol) containing protease inhibitor cocktail (#04693132001, Roche). .. Either 3 μg anti-ALK (#3633, CST), anti-SLC3A2 (#13180, CST), anti-phospho-Tyrosine (p-Y-1000, #8954, CST) or isotype control (#AB-105-C, R&D systems) antibody was added to 300 μg of cell lysate for overnight rotation at 4 °C, followed by incubation with 25 μl Protein G Sepharose 4 Fast Flow for an additional 2 h. Samples were washed twice with high salt IP buffer (500mM NaCl, 50 mM Tris-HCl, 1% Triton X-100, 1% glycerol) and then washed once with IP buffer (150mM NaCl, 50 mM Tris-HCl, 1% Triton X-100, 1% glycerol). ..

    Article Title: Anaplastic Lymphoma Kinase signaling stabilizes SLC3A2 expression via MARCH11 to promote neuroblastoma cell growth
    Article Snippet: NB1 cells were treated with ALKAL2 (1 μg/ml) or/and lorlatinib (30 nM) or/and MG132 (10 μM), CLB-GE and CLB-BAR cells were treated with lorlatinib (30 nM) for 24 h prior to lysis with IP buffer (150 mM NaCl, 50 mM Tris-HCl, 1% Triton X-100, 1% glycerol) containing protease inhibitor cocktail (#04693132001, Roche). .. Either 3 μg anti-ALK (#3633, CST), anti-SLC3A2 (#13180, CST), anti-phospho-Tyrosine (p-Y-1000, #8954, CST) or isotype control (#AB-105-C, R&D systems) antibody was added to 300 μg of cell lysate for overnight rotation at 4 °C, followed by incubation with 25 μl Protein G Sepharose 4 Fast Flow for an additional 2 h. Samples were washed twice with high salt IP buffer (500 mM NaCl, 50 mM Tris-HCl, 1% Triton X-100, 1% glycerol) and then washed once with IP buffer (150 mM NaCl, 50 mM Tris-HCl, 1% Triton X-100, 1% glycerol). ..



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